Identification of Alkaloid Compounds Arborinine and Graveoline from Ruta angustifolia (L.) Pers for their Antifungal Potential against Isocitrate lyase (ICL1) gene of Candida albicans

Candida albicans has been reported globally as the most widespread pathogenic species contributing candidiasis from superficial to systemic infections in immunocompromised individuals. Their metabolic adaptation depends on glyoxylate cycle survive nutrient-limited host. The long term usage of fungistatic drugs and lack cidal frequently result strains that could resist commonly used antifungals display multidrug resistance (MDR). In search potential therapeutic intervention novel fungicidals, we have explored a plant alkaloids, namely arborinine graveoline for its antifungal potential. Alkaloids belongs Rutaceae family with numerous antimicrobial activities. this study, aimed isolate identify active alkaloids R. angustifolia assess effect targeting C. isocitrate lyase (ICL) gene which regulates lyase, key enzyme virulence albicans. were extracted by bioassay guided isolation technique further identified TLC profile compared standard through HPLC NMR analysis. activities quantified means MIC (Minimum Inhibitory Concentration). expression targeted upon treatment was analysed using RT-qPCR western blot. Additionally, study looked at drug-likeness toxicity alkaloid compounds silico Spectroscopic analysis showed isolated characterized acridone, furoquinoline, 4-quinolone known graveoline. Results each compound significantly inhibited growth dose 250 500 µg/mL confirm activity. Each found successfully downregulate both ICL1 CaIcl1 protein. Finally, ADMET suggests good prediction chemical properties, absorption, distribution, metabolism, excretion (ADMET) will contribute drug discovery development later on.